01 April, 2019

Scorpion envenomations in South Africa during a 10-year period


South Africa harbors a large diversity of scorpions, including many species in the medical important genus Parabuthus Pocock, 1890 (Buthidae). Carine J. Marks and co-workers have recently published a retrospective analysis of the scorpion cases managed by the Tygerberg Poisons Information Centre over a 10 year period.

The main conclusion is that the incidence of severe scorpionism were low in this period. 65% of the sting cases had no or minor symptoms, mainly local pain. As usual, children may be more vulnerable and extra vigilance is needed in cases involving small children. The species involved in the study were usually not involved, but it is well known that Parabuthus granulatus (Ehrenberg, 1831) and P. transvaalicus (Purcell, 1899) are the most dangerous species in South Africa.

Abstract:
Introduction: South Africa has a wide distribution of scorpion species, yet limited data are available regarding the incidence and severity of scorpion envenomation. The aim of this study was to analyse South African epidemiological data of scorpion stings and envenomation as reported to the Tygerberg Poisons Information Centre (TPIC).
Methods: A retrospective analysis was conducted of scorpion-related telephonic consultations to the TPIC over a ten year period (1 January 2005 to 31 December 2014). Data were entered onto a Microsoft Excel® spreadsheet and descriptive statistics are presented for all variables. Associations with severity of envenomation are presented as odds ratios (OR) with 95% confidence intervals (95%CI).
Results: During the study period 52,163 consultations were processed by the TPIC of which 740 (1.4%) cases involved scorpion stings. Of these, 146 (19.7%) cases were deemed serious envenomations. Antivenom was recommended to be administered in 131 (90%) of these cases. Healthcare professionals made most calls (63%), but were less likely to phone for non-serious cases (OR 0.16; 95%CI 0.09 to 0.29). The Western Cape Province had the highest incidence of calls (6.9 scorpion-related calls/100 000 people). Adults (> 20 years) were victims in 71.4% of cases, and were more likely to experience less serious stings (OR 0.57; 95%CI 0.37 to 0.86). The TPIC was consulted within six hours of the sting occurring in 356 (48.1%) cases with a significant association to less severity (OR 3.51; 95%CI 1.9 to 6.3). Only 2% (15) of the scorpions were available for identification.
Conclusion: The incidence of severe scorpionism to the TPIC was low. Care should be taken when children are involved and when calls are received more than six hours after the sting. TPIC consultants as well as healthcare professionals working in semi-arid regions should be aware of these high risk populations.


Reference:
Marks CJ, Muller GJ, Sachno D, Reuter H, Wium CA, Du Plessis CE, et al. The epidemiology and severity of scorpion envenoming in South Africa as managed by the Tygerberg Poisons Information Centre over a 10-year period. African journal of emergency medicine : Revue Africaine de la Medecine d'Urgence. 2019;9(1):21-4. [Open Access]

Jaguajir rochae is able to perceive differences between small and large prey and make decisions regarding venom usage


Several studies have shown that scorpions have different strategies for optimizing the venom use. The reason for this is of course because it take times to renew the venom and it is costly as the venom is a mixture of complex proteins. This is often called the venom optimization hypothesis. This also means that optimizing venom usage might directly affect the predatory behavior and physiology of scorpions.

Meykson Alexandre da Silva and co-workers have recently published an article showing that the amount of venom available for individuals of Jaguajir rochae (Borelli, 1910) (Butidae) did have an effect on their prey capture behavior. Individuals with depleted venom glands did not attack large prey, but did try to catch smaller prey that could be handled with pedipalps only. The study shows that this scorpion is able to perceive differences between small and large prey and make decisions regarding venom usage. These results are in accordance with the the venom optimization hypothesis

Abstract:
Animal venom is composed of a complex mixture of protein-rich chemicals. Synthesis of animal venom incurs a high metabolic cost and is a prolonged process; consequently, animals use their venom cautiously and economically. Some studies have shown that venomous animals modulate the amount and/or type of venom used depending on certain factors, such as prey size or the intensity of predation threat. Here, we investigated how the quantity of venom that is available for use by the scorpion Jaguajir rochae interferes with its choice of prey.We used two types of prey of contrasting size (small 200–300-mg and large 600–700-mg cockroaches). The results showed that the amount of venom influences the feeding behavior of this species. Most scorpions without venom exhibited a low interest when large prey was present, but frequently attacked small prey. The scorpions also showed a distinct pattern in the time between venom extraction and the initiation of hunting behavior. In conclusion, J. rochae is able to perceive differences between small and large prey and make decisions regarding venom usage, supporting the "venom optimization hypothesis" (or "venom metering hypothesis"), by minimizing the venom use due to it being an energetically expensive resource.

Reference:
Silva MA, Silvia NA, Lira AFA, Martins RD. Role of venom quantity in the feeding behavior of Jaguajir rochae (Scorpiones: Buthidae). Acta Ethologica. 2019;Published Online 09 March 2019. [Subscription required for full text]

Thanks to Meykson Alexandre da Silva for sending me their interesting article which confirm some of the assumptions that I did in my old studies of sting use in scorpions.

29 March, 2019

A new species of Chaerilus from Vietnam



Wilson Lourenco has recently published a new species of Chaerilus Simon, 1877 (Chaerilidae) from Vietnam.

Chaerilus honba Lourenco, 2019

Abstract:


Reference:
Lourenco WR. The genus Chaerilus Simon, 1877 (Scorpiones: Chaerilidae) in Vietnam, with the description of a new species. Arachnology. 2019;18(1):32-6.

Family Chaerilidae

28 March, 2019

A new phylogenomic study reassessing the relationships of Vaejovidae and a new high-level classification of Scorpiones


The higher-level systematics of scorpions has been significantly revised and discussed in the recent year, not without controversies.The family division chosen in The Scorpion Files are not accepted by all researchers, but fortunately more research are being done making the picture more clearly. There may be changes in the family structure in The scorpion Files in the time to come when I have the time to review recent contributions on higher-level systematics.

Carlos E. Santibáñez-López and co-workers have recently published a study  reassessing the relationships of Vaejovidae and a new high-level classification of Scorpiones. According to co-author Prashant Sharma, the main conclusions are:

1. As presently defined, Vaejovidae is diphyletic (Uroctonus are not true vaejovids).

2. The true Vaejovidae (excluding Uroctonus) are the sister group of Scorpionoidea.

3. To accommodate this tree topology, the study established two  superfamilies to redress the paraphyly of Chactoidea: Vaejovoidea and Superstitionoidea.

The scorpion Files doesn't list higher-level systematics, but it is important to note that status of Uroctonus Thorell, 1876 has to be set to incertae sedis because it doesn't belong to either Vaejovidae or Chactidae. More studies are necessary before its family placement can be decided. Until this happens, I keep the genus in Chactidae.

Abstract:
The Neartic family Vaejovidae (Scorpiones: Chactoidea) has long been treated as a diverse and systematically cohesive group of scorpions, but its monophyly and relationship to other scorpion families have historically been questioned. Morphological data have supported its monophyly and a variety of phylogenetic placements within the superfamily Chactoidea. Recent phylogenomic analyses have instead recovered vaejovids as polyphyletic (albeit with minimal taxonomic sampling) and Chactoidea as paraphyletic. Here, we reexamined the monophyly and phylogenetic placement of the family Vaejovidae, sampling 17 new vaejovid libraries using high throughput transcriptomic sequencing. Our phylogenomic analyses revealed a previous misplacement of Smeringurus mesaensis. Regardless, we recovered Vaejovidae as diphyletic due to the placement of the enigmatic genus Uroctonus. The remaining vaejovids formed a clade that was strongly supported as the sister group of the superfamily Scorpionoidea, a placement insensitive to matrix completeness or concatenation vs. species tree approaches to inferring the tree topology. Chactoidea was invariably recovered as a paraphyletic group due to the nested placement of Scorpionoidea. As first steps to resolving the paraphyly of Chactoidea, we take the following systematic actions: (1) we establish the superfamily Superstitionoidea (new superfamily) to accommodate Superstitioniidae; (2) we restore Vaejovoidea (status revalidated) as a valid superfamily that excludes Uroctonus; and (3) we treat the families Caraboctonidae, Troglotayosicidae, and the subfamily Uroctoninae as incertae sedis with respect to superfamilial placement. Our systematic actions thus establish the monophyly of the presently redefined Chactoidea and Vaejovoidea.

Reference:Santibanez-Lopez C, Gonzalez-Santillan E, Monod L, Sharma PP. Phylogenomics facilitates stable scorpion systematics: Reassessing the relationships of Vaejovidae and a new high-level classification of Scorpiones (Arachnida). Mol Phylogenet Evol. 2019;135:22-30. [Subscription required for full text]

Thanks to Dr. Prashant Sharma for sending me this article and for explaining to me the main points of this study. Thanks also to Carlos E. Santibáñez-López for sending me the paper.

14 March, 2019

Cytogenetic and molecular approaches reveal cryptic diversity in Alpine scorpions in the genus Euscorpius


Phenotypic conservatism is typical for many scorpion taxa.In layman's term, this mean that many scorpion species look morphologically similar making it almost impossible to separate them by traditional taxonomical methods. The European genus Euscorpius Thorell, 1876 (Euscorpiidae) is an excellent example of this. Originally this genus contained less than then species, while today it contains no less than 64 species.

And it seems that there is still more to come. By using a new combination of cytogenetic and molecular methods, Jana Štundlováa and co-workers have recently published a study of the three Alpine species of the subgenus Euscorpius (Alpiscorpius): E. (A.) alpha, E. (A.) germanus, and E. (A.) gamma.

Their study reveals a cryptic diversity in the populations of the Alpine species, with several "races" (highly distinct karyotypic races) within each species. These races had discrete geographical distributions. Even though these results clearly shows several independent taxa, a thorough taxonomical revision with morphological studies of the subgenus is necessary before any taxonomical decisions can be made.

Some of the methods used in this study are new and open new possibilities for our understanding of the species diversity of scorpions.

Abstract:
Over time, mountain biota has undergone complex evolutionary histories that have left imprints on its genomic arrangement, geographical distribution and diversity of contemporary lineages. Knowledge on these biogeographical aspects still lags behind for invertebrates inhabiting the Alpine region. In the present study, we examined three scorpion species of the subgenus Euscorpius (Alpiscorpius) from the European Alps using cytogenetic and molecular phylogenetic approaches to determine the variation and population structure of extant lineages at both chromosome and genetic level, and to provide an insight into the species diversification histories. We detected considerable intraspecific variability in chromosome complements and localization of the 18S rDNA loci in all studied species. Such chromosome differences were noticeable as the existence of three [in E. (A.) alpha and E. (A.) germanus] or four [in E. (A.) gamma] range-restricted karyotypic races. These races differed from one another either by 2n [in E. (A.) alpha 2n=54, 60, 90; in E. (A.) gamma 2n=58, 60, 88, 86–92], or by the karyotypic formula [in E. (A.) germanus 2n=34m+12sm; 36m+10sm; 42m+4sm]. Using mitochondrial (16S rRNA, COI) and nuclear (28S rDNA) genetic markers, we examined genetic variation and reconstructed phylogenetic relationships among the karyotypic races. Both approaches provided evidence for the existence of ten deeply divergent lineages exhibiting the features of local endemics and indicating the presence of cryptic species. Molecular dating analyses suggest that these lineages diversified during the Plio-Pleistocene and this process was presumably accompanied by dynamic structural changes in the genome organization.

Reference:
Stundlova J, Smid J, Nguyen P, Stahlavsky F. Cryptic diversity and dynamic chromosome evolution in Alpine scorpions (Euscorpiidae: Euscorpius). Mol Phylogenet Evol. 2019;134:152-63. [Subscription required for full text]

Thanks to Victor Fet and Frantisek Kovarik for sending me this interesting article!

Distribution and habitat of Euscorpius carpathicus in Romania


Euscorpius carpathicus (Linnaeus, 1767) (Euscorpiidae) was previously distributed all over Europe, but modern taxonomy revealed that it was a major species complex hiding many unique species. Today, E. carpathicus is limited to Romania only.

Severus-Daniel Covaciu-Marcov & Sára Ferenţi have recently published an article on the distribution of E. carpathicus in parts on Romania. Data on habitat is also presented.

Abstract:
In 2016-2018, we identified 48 distribution records of Euscorpius carpathicus in the Cozia National Park, from the Romanian Southern Carpathians. The Carpathian scorpion was found between 300 and 847 m a.s.l., in forested regions, being more numerous in the lower areas situated along the Olt River. E. carpathicus is a native species in the region; it populates natural areas with low human impact.

Reference:
Covaciu-Marcov S-D, Ferenti S. An Endemic Species in a Protected Area: Euscorpius carpathicus (L., 1767) in the Cozia National Park, Romania (Scorpiones: Euscorpiidae). Euscorpius. 2019(279):1-6. [Open Access]

Family Euscorpiidae

28 February, 2019

New Orthochirus species from The Middle East


Frantisek Kovarik and co-workers have recently published a revision of the genus Orthochirus Karsch, 1891 (Buthidae) from Turkey, Iraq and Iran. Three new species are described and one species is elevated from subspecies status.

Orthochirus fomichevi Kovarik, Yagmur, Fet & Hussen, 2019 (New species from Turkey & Iraq)

Orthochirus gantenbeini Kovarik, Yagmur, Fet & Hussen, 2019 (New species from Iran)

Orthochirus navidpouri Kovarik, Yagmur, Fet & Hussen, 2019 (New species from Iran)

Orthochirus mesopotamicus Birula, 1918 (Elevated to species status. Previous status: Orthochirus scrobiculosus mesopotamicus Birula, 1918)

The article has an identification key for the species in the region.

Abstract:
Three new species, Orthochirus fomichevi sp. n. from Turkey and Iraq, O. gantenbeini sp. n. from Iran (Khoozestan Province), and O. navidpouri sp. n. from Iran (Khoozestan and Lorestan Provinces) are described, compared with other Orthochirus species from the region, and fully illustrated with color photos. Lectotype of O. mesopotamicus Birula, 1918 stat. n. from Iran (Khoozestan Province) is designated. Emended diagnoses are given for O. iranus Kovařík, 2004, O. iraqus Kovařík, 2004, O. mesopotamicus Birula, 1918 stat. n., and O. zagrosensis Kovařík, 2004. A key and a distribution map are included.

Reference:
Kovarik F, Yagmur EA, Fet V, Hussen FS. A review of Orthochirus from Turkey, Iraq, and Iran (Khoozestan, Ilam, and Lorestan Provinces), with descriptions of three new species (Scorpiones: Buthidae). Euscorpius. 2019(278):1-31. [Open Access]

Family Buthidae